The Science of Proteomics

NB. This course has now been withdrawn.

Course provider:
The University of Leeds

Course contact:
Rob Garbutt (rob@ukgrid.net)

Summary:

On completion of this module, students should be able to:

Appreciate the fundamental aspects of a new scientific approach known as Proteomics. They should appreciate the experimental aspects which lead to the identification of the "proteome" of a system and be knowledgeable in the bioinformatic approaches that allow the protein content of a system to be determined. They should have some practical experience of these bioinformatic aspects and be able to draw conclusions from the experimental results.

Syllabus:

The course is divided into 10 section as follows:-

1. Protein structure and post-translational modifications
2. An introduction to proteomics
3. An exploration of the choice of material, the handling and storage of samples and the statistical analysis of samples
4. Sample preparation and fractionation techniques
5. A look at HPLC and MudPIT
6. A detailed look at 2-DE
7. An introduction to mass spectrometry looking at the different methods available and how these benefit proteomics
8. Bioinformatics and data analysis
9. Data storage, interchange and retrieval
10. Introduction to Protein Interactions

Further details:
The assessment elements:

for the majority of sections of the course there will be one tutorial exercise which must be completed satisfactorily;

there will be one Java simulation, two written assessments and assessment on contributions to group work through the bulletin board.

Assessment weightings

The final grading for the module will be a weighted mean of the marks for the following assessments.

Assessment 1. Protein Identification Java Simulation, 30%
Assessment 2. Data Storage, Interchange and Retrieval Essay, 20%
Assessment 3. Protein-Protein Interaction Website Design, 20%
Assessment 4. Contributions to Group Work via the Bulletin Board, 30%

Technical requirements:
This module is delivered entirely via the internet, so a reliable internet connection is essential.
You will need access to a PC running a recent version of Microsoft Windows (preferably Windows XP or Windows 2000), with a screen resolution of 1024 x 768.

No browser other than Microsoft Internet Explorer is currently recommended for this module.
In addition to Microsoft Internet Explorer, you will need to download and install the following plug-ins:

Adobe SVG Viewer.
Sun Java plug-in which supports Java 1.4.

References:

Sam Hanash, Disease Proteomics, Nature, 422, PageNumber (226)
M. Tyers & M. Mann, From Genomics to Proteomics, Nature, 422, PageNumber (193)

Course provider:	The University of Leeds
Course contact:	Rob Garbutt (rob@ukgrid.net)
Summary:	On completion of this module, students should be able to: Appreciate the fundamental aspects of a new scientific approach known as Proteomics. They should appreciate the experimental aspects which lead to the identification of the "proteome" of a system and be knowledgeable in the bioinformatic approaches that allow the protein content of a system to be determined. They should have some practical experience of these bioinformatic aspects and be able to draw conclusions from the experimental results.
Syllabus:	The course is divided into 10 section as follows:- 1. Protein structure and post-translational modifications 2. An introduction to proteomics 3. An exploration of the choice of material, the handling and storage of samples and the statistical analysis of samples 4. Sample preparation and fractionation techniques 5. A look at HPLC and MudPIT 6. A detailed look at 2-DE 7. An introduction to mass spectrometry looking at the different methods available and how these benefit proteomics 8. Bioinformatics and data analysis 9. Data storage, interchange and retrieval 10. Introduction to Protein Interactions
Further details:	The assessment elements: for the majority of sections of the course there will be one tutorial exercise which must be completed satisfactorily; there will be one Java simulation, two written assessments and assessment on contributions to group work through the bulletin board. Assessment weightings The final grading for the module will be a weighted mean of the marks for the following assessments. Assessment 1. Protein Identification Java Simulation, 30% Assessment 2. Data Storage, Interchange and Retrieval Essay, 20% Assessment 3. Protein-Protein Interaction Website Design, 20% Assessment 4. Contributions to Group Work via the Bulletin Board, 30%
Technical requirements:	This module is delivered entirely via the internet, so a reliable internet connection is essential. You will need access to a PC running a recent version of Microsoft Windows (preferably Windows XP or Windows 2000), with a screen resolution of 1024 x 768. No browser other than Microsoft Internet Explorer is currently recommended for this module. In addition to Microsoft Internet Explorer, you will need to download and install the following plug-ins: Adobe SVG Viewer. Sun Java plug-in which supports Java 1.4.
References:	Sam Hanash, Disease Proteomics, Nature, 422, PageNumber (226) M. Tyers & M. Mann, From Genomics to Proteomics, Nature, 422, PageNumber (193)

Back to module page

2 July 2009 by Heather Vincent